Laboratory Techniques in Biochemistry and Molecular Biology Vol 10: DNA Sequencing

This volume provides a comprehensive description of the principles and methods used in DNA sequencing. Following a detailed introduction the chapters are: DNA sequencing; Chain terminator sequencing; Primed synthesis methods applied to DNA fragments cloned into phage M13; DNA sequencing by the Maxam-Gilbert chemical procedure; Computer methods for DNA sequencers; Appendices including contractions and special terms, cloning vectors, commercially available restriction endonucleases, and autoradiography.